Saunders Bimolecular complementation affinity purification (BiCAP) reveals dimer-specific protein interactions for ERBB2 dimers. Shearer, Jessie McKenna, Adrian Wan, Joseph Lau, Samuel Aparicio, Darren N. doi: 10.1093/nar/gks941īenedetta Turriziani, Amaya Garcia-Munoz, Ruth Pilkington, Cinzia Raso, Walter Kolch, Alexander von Kriegsheim On-Beads Digestion in Conjunction with Data-Dependent Mass Spectrometry: A Shortcut to Quantitative and Dynamic Interaction Proteomics. Hyman, Michiel Vermeulen Stoichiometry of chromatin-associated protein complexes revealed by label-free quantitative mass spectrometry-based proteomics. Glover, Vincent Archambault Affinity Purification of Protein Complexes from Drosophila Embryos in Cell Cycle Studies. Dzhindzhev, Pier Paolo D’Avino, Marcin R.
Zoltan Lipinszki, Peng Wang, Rhys Grant, Catherine Lindon, Nikola S. Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes. Morrice, Sam Swift, Ulrich Rothbauer, Heinrich Leonhardt, Angus Lamond. Laura Trinkle-Mulcahy, Séverine Boulon, Yun Wah Lam, Roby Urcia, François-Michel Boisvert, Franck Vandermoere, Nick A. Complete tryptic digest results in 4-5 peptides. The GFP-Trap is optimized for on-bead digestion.
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis. Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragmentīead size: ~ 90 µm (cross-linked 4 % agarose beads) YFP, Citrine, eCitrine, eYFP, Venus, Ypetįor complete list, please click here: Fluorescent protein specificity tableġ0 µL slurry bind about 12 µg of recombinant GFP AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K